H2N2V1, Main, Exploration, bibRecord, 000B80

Broad humoral and cellular immunity elicited by a bivalent DNA vaccine encoding HA and NP genes from an H5N1 virus.

Identifieur interne : 000B80 ( Main/Exploration ); précédent : 000B79; suivant : 000B81

Broad humoral and cellular immunity elicited by a bivalent DNA vaccine encoding HA and NP genes from an H5N1 virus.

Auteurs : Ke Xu [République populaire de Chine] ; Zhi-Yang Ling [République populaire de Chine] ; Liang Sun [République populaire de Chine] ; Ying Xu [République populaire de Chine] ; Chao Bian [République populaire de Chine] ; Yuan He [République populaire de Chine] ; Wei Lu [République populaire de Chine] ; Ze Chen [République populaire de Chine] ; Bing Sun [République populaire de Chine]

Source :

Viral Immunology [ 0882-8245 ] ; 2011-02.

RBID : Hal:pasteur-00622908

Abstract

Influenza A virus is highly variable and a major viral respiratory pathogen that can cause severe illness in humans. Therefore it is important to induce a sufficient immune response specific to current strains and to heterosubtypic viruses with vaccines. In this study, we developed a dual-promoter-based bivalent DNA vaccine that encodes both hemagglutinin (HA) and nucleoprotein (NP) proteins from a highly pathogenic A/Chicken/Henan/12/2004 (H5N1) virus. Our results show that the expression levels of HA and NP genes from the dual-promoter plasmid are similar to those seen when they are expressed individually in independent plasmids. When the bivalent DNA vaccine was inoculated via intramuscular injection and in vivo electroporation, high levels of both humoral and cellular immune responses were elicited against homologous H5N1 virus and heterosubtypic H9N2 virus. Furthermore, no obvious antigenic competition was observed between HA and NP proteins in the dual-promoter-based bivalent vaccine compared to monovalent vaccines. Our data suggest that a combination of influenza surface and internal viral genes in a dual-promoter-expressing plasmid may provide a new approach for developing a DNA vaccine that may protect not only specifically against a currently circulating strain, but also may cross-protect broadly against new heterosubtypic viruses.

Url:

https://hal-riip.archives-ouvertes.fr/pasteur-00622908

DOI: 10.1089/vim.2010.0056

Affiliations:

République populaire de Chine

Links toward previous steps (curation, corpus...)

to stream Hal, to step Corpus: 000028
to stream Hal, to step Curation: 000028
to stream Hal, to step Checkpoint: 000236
to stream Main, to step Merge: 000B84
to stream Main, to step Curation: 000B80

Le document en format XML

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          <desc>            <address>              <addrLine>225 South Chongqing Road, Shanghai 200025</addrLine>
              <country key="CN"></country>
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            <ref type="url">http://english.shanghaipasteur.cas.cn/</ref>
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<tutelle active="#struct-301247" type="indirect"><org type="regroupinstitution" xml:id="struct-301247" status="VALID">          <orgName>Réseau International des Instituts Pasteur</orgName>
          <orgName type="acronym">RIIP</orgName>
          <desc>            <address>              <addrLine>25, rue du Dr Roux 75724 Paris Cedex 15</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">https://research.pasteur.fr/ip-network</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>République populaire de Chine</country>
</affiliation>
</author>
<author><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-168194" status="INCOMING">          <orgName>Shanghai Institute of Biological Products</orgName>
          <desc>            <address>              <country key="CN"></country>
            </address>
          </desc>
          <listRelation>            <relation active="#struct-331766" type="direct"></relation>
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        <tutelles><tutelle active="#struct-331766" type="direct"><org type="institution" xml:id="struct-331766" status="INCOMING">          <orgName>China</orgName>
          <desc>            <address>              <country key="FR"></country>
            </address>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>République populaire de Chine</country>
</affiliation>
</author>
<author><name sortKey="Sun, Bing" sort="Sun, Bing" uniqKey="Sun B" first="Bing" last="Sun">Bing Sun</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-168263" status="VALID">          <orgName>Key Laboratory of Molecular Virology & Immunology</orgName>
          <orgName type="acronym">LMVI</orgName>
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            </address>
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          <orgName type="acronym">IPS-CAS</orgName>
          <desc>            <address>              <addrLine>225 South Chongqing Road, Shanghai 200025</addrLine>
              <country key="CN"></country>
            </address>
            <ref type="url">http://english.shanghaipasteur.cas.cn/</ref>
          </desc>
          <listRelation>            <relation active="#struct-301247" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle active="#struct-301247" type="indirect"><org type="regroupinstitution" xml:id="struct-301247" status="VALID">          <orgName>Réseau International des Instituts Pasteur</orgName>
          <orgName type="acronym">RIIP</orgName>
          <desc>            <address>              <addrLine>25, rue du Dr Roux 75724 Paris Cedex 15</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">https://research.pasteur.fr/ip-network</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>République populaire de Chine</country>
</affiliation>
</author>
</analytic>
<idno type="DOI">10.1089/vim.2010.0056</idno>
<series><title level="j">Viral Immunology</title>
<idno type="ISSN">0882-8245</idno>
<imprint><date type="datePub">2011-02</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
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<front><div type="abstract" xml:lang="en">              <p>Influenza A virus is highly variable and a major viral respiratory pathogen that can cause severe illness in humans. Therefore it is important to induce a sufficient immune response specific to current strains and to heterosubtypic viruses with vaccines. In this study, we developed a dual-promoter-based bivalent DNA vaccine that encodes both hemagglutinin (HA) and nucleoprotein (NP) proteins from a highly pathogenic A/Chicken/Henan/12/2004 (H5N1) virus. Our results show that the expression levels of HA and NP genes from the dual-promoter plasmid are similar to those seen when they are expressed individually in independent plasmids. When the bivalent DNA vaccine was inoculated via intramuscular injection and in vivo electroporation, high levels of both humoral and cellular immune responses were elicited against homologous H5N1 virus and heterosubtypic H9N2 virus. Furthermore, no obvious antigenic competition was observed between HA and NP proteins in the dual-promoter-based bivalent vaccine compared to monovalent vaccines. Our data suggest that a combination of influenza surface and internal viral genes in a dual-promoter-expressing plasmid may provide a new approach for developing a DNA vaccine that may protect not only specifically against a currently circulating strain, but also may cross-protect broadly against new heterosubtypic viruses.</p>
            </div>
</front>
</TEI>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
</list>
<tree><country name="République populaire de Chine"><noRegion><name sortKey="Xu, Ke" sort="Xu, Ke" uniqKey="Xu K" first="Ke" last="Xu">Ke Xu</name>
</noRegion>
<name sortKey="Bian, Chao" sort="Bian, Chao" uniqKey="Bian C" first="Chao" last="Bian">Chao Bian</name>
<name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name>
<name sortKey="He, Yuan" sort="He, Yuan" uniqKey="He Y" first="Yuan" last="He">Yuan He</name>
<name sortKey="Ling, Zhi Yang" sort="Ling, Zhi Yang" uniqKey="Ling Z" first="Zhi-Yang" last="Ling">Zhi-Yang Ling</name>
<name sortKey="Lu, Wei" sort="Lu, Wei" uniqKey="Lu W" first="Wei" last="Lu">Wei Lu</name>
<name sortKey="Sun, Bing" sort="Sun, Bing" uniqKey="Sun B" first="Bing" last="Sun">Bing Sun</name>
<name sortKey="Sun, Liang" sort="Sun, Liang" uniqKey="Sun L" first="Liang" last="Sun">Liang Sun</name>
<name sortKey="Xu, Ying" sort="Xu, Ying" uniqKey="Xu Y" first="Ying" last="Xu">Ying Xu</name>
</country>
</tree>
</affiliations>
</record>

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Broad humoral and cellular immunity elicited by a bivalent DNA vaccine encoding HA and NP genes from an H5N1 virus.

Broad humoral and cellular immunity elicited by a bivalent DNA vaccine encoding HA and NP genes from an H5N1 virus.

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